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crl 11268 oligonucleotides si rna munc13 2  (ATCC)
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Crl 11268 Oligonucleotides Si Rna Munc13 2, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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unc13a  (Proteintech)
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TBI-related neurodegenerative phenotypes emerge 7 days after injury of cBOs (A–C) Representative WB (A) and quantification of TDP-43 (B) and pTau (AT8, C) in detergent-soluble (i.e. radio-immunoprecipitation assay [RIPA] buffer) and urea-soluble fractions from cBOs 7 days after blast exposure. Student’s t test, n = 4 in (B) and n = 5 in (C). Ponceau staining was used to normalize protein loading. (D–G) Representative WB and quantifications of STMN2 (D and E) and <t>UNC13A</t> (F and G) levels from total protein extracts of cBOs 7 days after blast exposure. GAPDH was used as loading control. Student’s t test, n = 8 in (E), n = 6 in (G). (H and I) Representative immunofluorescence images of TBI or sham cBOs 7 days after injury stained for UNC13A (green), TDP-43 (gray), and MAP2 (red). A representative MAP2-positive cell is shown in the inset, highlighting a shift in TDP-43 distribution from strictly nuclear in the sham control to cytoplasmic and aggregating after TBI. Magnification: 63x; scale bars: 15 μm in the main panels and 5 μm in zoomed in images. (J and K) Quantification of TDP-43 mean fluorescence intensity (MFI) in the nucleus ( n = 25) (J) and the correlation between normalized nuclear TDP-43 levels and normalized UNC13A levels (K). (L and O) Representative WB ( L ) and quantification of cryptic exon (CE)-containing HDGFL2 isoform ( n = 4) (M), HDGFL2 levels ( n = 6 for sham and n = 4 for TBI) (N), and their ratio ( n = 4) (O) in cBO 7 days after blast injury. GAPDH was used as loading control. For all, bars represent mean ± standard deviation, while circles and triangles indicate individual experiments using the two independent iPSC lines; ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001.
Unc13a, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit  (Proteintech)
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TBI-related neurodegenerative phenotypes emerge 7 days after injury of cBOs (A–C) Representative WB (A) and quantification of TDP-43 (B) and pTau (AT8, C) in detergent-soluble (i.e. radio-immunoprecipitation assay [RIPA] buffer) and urea-soluble fractions from cBOs 7 days after blast exposure. Student’s t test, n = 4 in (B) and n = 5 in (C). Ponceau staining was used to normalize protein loading. (D–G) Representative WB and quantifications of STMN2 (D and E) and <t>UNC13A</t> (F and G) levels from total protein extracts of cBOs 7 days after blast exposure. GAPDH was used as loading control. Student’s t test, n = 8 in (E), n = 6 in (G). (H and I) Representative immunofluorescence images of TBI or sham cBOs 7 days after injury stained for UNC13A (green), TDP-43 (gray), and MAP2 (red). A representative MAP2-positive cell is shown in the inset, highlighting a shift in TDP-43 distribution from strictly nuclear in the sham control to cytoplasmic and aggregating after TBI. Magnification: 63x; scale bars: 15 μm in the main panels and 5 μm in zoomed in images. (J and K) Quantification of TDP-43 mean fluorescence intensity (MFI) in the nucleus ( n = 25) (J) and the correlation between normalized nuclear TDP-43 levels and normalized UNC13A levels (K). (L and O) Representative WB ( L ) and quantification of cryptic exon (CE)-containing HDGFL2 isoform ( n = 4) (M), HDGFL2 levels ( n = 6 for sham and n = 4 for TBI) (N), and their ratio ( n = 4) (O) in cBO 7 days after blast injury. GAPDH was used as loading control. For all, bars represent mean ± standard deviation, while circles and triangles indicate individual experiments using the two independent iPSC lines; ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001.
Rabbit, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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munc13 4  (Proteintech)
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TBI-related neurodegenerative phenotypes emerge 7 days after injury of cBOs (A–C) Representative WB (A) and quantification of TDP-43 (B) and pTau (AT8, C) in detergent-soluble (i.e. radio-immunoprecipitation assay [RIPA] buffer) and urea-soluble fractions from cBOs 7 days after blast exposure. Student’s t test, n = 4 in (B) and n = 5 in (C). Ponceau staining was used to normalize protein loading. (D–G) Representative WB and quantifications of STMN2 (D and E) and <t>UNC13A</t> (F and G) levels from total protein extracts of cBOs 7 days after blast exposure. GAPDH was used as loading control. Student’s t test, n = 8 in (E), n = 6 in (G). (H and I) Representative immunofluorescence images of TBI or sham cBOs 7 days after injury stained for UNC13A (green), TDP-43 (gray), and MAP2 (red). A representative MAP2-positive cell is shown in the inset, highlighting a shift in TDP-43 distribution from strictly nuclear in the sham control to cytoplasmic and aggregating after TBI. Magnification: 63x; scale bars: 15 μm in the main panels and 5 μm in zoomed in images. (J and K) Quantification of TDP-43 mean fluorescence intensity (MFI) in the nucleus ( n = 25) (J) and the correlation between normalized nuclear TDP-43 levels and normalized UNC13A levels (K). (L and O) Representative WB ( L ) and quantification of cryptic exon (CE)-containing HDGFL2 isoform ( n = 4) (M), HDGFL2 levels ( n = 6 for sham and n = 4 for TBI) (N), and their ratio ( n = 4) (O) in cBO 7 days after blast injury. GAPDH was used as loading control. For all, bars represent mean ± standard deviation, while circles and triangles indicate individual experiments using the two independent iPSC lines; ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001.
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rabbit anti unc13a  (Proteintech)
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TBI-related neurodegenerative phenotypes emerge 7 days after injury of cBOs (A–C) Representative WB (A) and quantification of TDP-43 (B) and pTau (AT8, C) in detergent-soluble (i.e. radio-immunoprecipitation assay [RIPA] buffer) and urea-soluble fractions from cBOs 7 days after blast exposure. Student’s t test, n = 4 in (B) and n = 5 in (C). Ponceau staining was used to normalize protein loading. (D–G) Representative WB and quantifications of STMN2 (D and E) and <t>UNC13A</t> (F and G) levels from total protein extracts of cBOs 7 days after blast exposure. GAPDH was used as loading control. Student’s t test, n = 8 in (E), n = 6 in (G). (H and I) Representative immunofluorescence images of TBI or sham cBOs 7 days after injury stained for UNC13A (green), TDP-43 (gray), and MAP2 (red). A representative MAP2-positive cell is shown in the inset, highlighting a shift in TDP-43 distribution from strictly nuclear in the sham control to cytoplasmic and aggregating after TBI. Magnification: 63x; scale bars: 15 μm in the main panels and 5 μm in zoomed in images. (J and K) Quantification of TDP-43 mean fluorescence intensity (MFI) in the nucleus ( n = 25) (J) and the correlation between normalized nuclear TDP-43 levels and normalized UNC13A levels (K). (L and O) Representative WB ( L ) and quantification of cryptic exon (CE)-containing HDGFL2 isoform ( n = 4) (M), HDGFL2 levels ( n = 6 for sham and n = 4 for TBI) (N), and their ratio ( n = 4) (O) in cBO 7 days after blast injury. GAPDH was used as loading control. For all, bars represent mean ± standard deviation, while circles and triangles indicate individual experiments using the two independent iPSC lines; ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001.
Rabbit Anti Unc13a, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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55053 1 ap rrid ab 10804173  (Proteintech)
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TBI-related neurodegenerative phenotypes emerge 7 days after injury of cBOs (A–C) Representative WB (A) and quantification of TDP-43 (B) and pTau (AT8, C) in detergent-soluble (i.e. radio-immunoprecipitation assay [RIPA] buffer) and urea-soluble fractions from cBOs 7 days after blast exposure. Student’s t test, n = 4 in (B) and n = 5 in (C). Ponceau staining was used to normalize protein loading. (D–G) Representative WB and quantifications of STMN2 (D and E) and <t>UNC13A</t> (F and G) levels from total protein extracts of cBOs 7 days after blast exposure. GAPDH was used as loading control. Student’s t test, n = 8 in (E), n = 6 in (G). (H and I) Representative immunofluorescence images of TBI or sham cBOs 7 days after injury stained for UNC13A (green), TDP-43 (gray), and MAP2 (red). A representative MAP2-positive cell is shown in the inset, highlighting a shift in TDP-43 distribution from strictly nuclear in the sham control to cytoplasmic and aggregating after TBI. Magnification: 63x; scale bars: 15 μm in the main panels and 5 μm in zoomed in images. (J and K) Quantification of TDP-43 mean fluorescence intensity (MFI) in the nucleus ( n = 25) (J) and the correlation between normalized nuclear TDP-43 levels and normalized UNC13A levels (K). (L and O) Representative WB ( L ) and quantification of cryptic exon (CE)-containing HDGFL2 isoform ( n = 4) (M), HDGFL2 levels ( n = 6 for sham and n = 4 for TBI) (N), and their ratio ( n = 4) (O) in cBO 7 days after blast injury. GAPDH was used as loading control. For all, bars represent mean ± standard deviation, while circles and triangles indicate individual experiments using the two independent iPSC lines; ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001.
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rabbit anti-munc13 synaptic systems 126103  (Synaptic Systems)
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TBI-related neurodegenerative phenotypes emerge 7 days after injury of cBOs (A–C) Representative WB (A) and quantification of TDP-43 (B) and pTau (AT8, C) in detergent-soluble (i.e. radio-immunoprecipitation assay [RIPA] buffer) and urea-soluble fractions from cBOs 7 days after blast exposure. Student’s t test, n = 4 in (B) and n = 5 in (C). Ponceau staining was used to normalize protein loading. (D–G) Representative WB and quantifications of STMN2 (D and E) and <t>UNC13A</t> (F and G) levels from total protein extracts of cBOs 7 days after blast exposure. GAPDH was used as loading control. Student’s t test, n = 8 in (E), n = 6 in (G). (H and I) Representative immunofluorescence images of TBI or sham cBOs 7 days after injury stained for UNC13A (green), TDP-43 (gray), and MAP2 (red). A representative MAP2-positive cell is shown in the inset, highlighting a shift in TDP-43 distribution from strictly nuclear in the sham control to cytoplasmic and aggregating after TBI. Magnification: 63x; scale bars: 15 μm in the main panels and 5 μm in zoomed in images. (J and K) Quantification of TDP-43 mean fluorescence intensity (MFI) in the nucleus ( n = 25) (J) and the correlation between normalized nuclear TDP-43 levels and normalized UNC13A levels (K). (L and O) Representative WB ( L ) and quantification of cryptic exon (CE)-containing HDGFL2 isoform ( n = 4) (M), HDGFL2 levels ( n = 6 for sham and n = 4 for TBI) (N), and their ratio ( n = 4) (O) in cBO 7 days after blast injury. GAPDH was used as loading control. For all, bars represent mean ± standard deviation, while circles and triangles indicate individual experiments using the two independent iPSC lines; ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001.
Rabbit Anti Munc13 Synaptic Systems 126103, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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TBI-related neurodegenerative phenotypes emerge 7 days after injury of cBOs (A–C) Representative WB (A) and quantification of TDP-43 (B) and pTau (AT8, C) in detergent-soluble (i.e. radio-immunoprecipitation assay [RIPA] buffer) and urea-soluble fractions from cBOs 7 days after blast exposure. Student’s t test, n = 4 in (B) and n = 5 in (C). Ponceau staining was used to normalize protein loading. (D–G) Representative WB and quantifications of STMN2 (D and E) and <t>UNC13A</t> (F and G) levels from total protein extracts of cBOs 7 days after blast exposure. GAPDH was used as loading control. Student’s t test, n = 8 in (E), n = 6 in (G). (H and I) Representative immunofluorescence images of TBI or sham cBOs 7 days after injury stained for UNC13A (green), TDP-43 (gray), and MAP2 (red). A representative MAP2-positive cell is shown in the inset, highlighting a shift in TDP-43 distribution from strictly nuclear in the sham control to cytoplasmic and aggregating after TBI. Magnification: 63x; scale bars: 15 μm in the main panels and 5 μm in zoomed in images. (J and K) Quantification of TDP-43 mean fluorescence intensity (MFI) in the nucleus ( n = 25) (J) and the correlation between normalized nuclear TDP-43 levels and normalized UNC13A levels (K). (L and O) Representative WB ( L ) and quantification of cryptic exon (CE)-containing HDGFL2 isoform ( n = 4) (M), HDGFL2 levels ( n = 6 for sham and n = 4 for TBI) (N), and their ratio ( n = 4) (O) in cBO 7 days after blast injury. GAPDH was used as loading control. For all, bars represent mean ± standard deviation, while circles and triangles indicate individual experiments using the two independent iPSC lines; ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001.
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TBI-related neurodegenerative phenotypes emerge 7 days after injury of cBOs (A–C) Representative WB (A) and quantification of TDP-43 (B) and pTau (AT8, C) in detergent-soluble (i.e. radio-immunoprecipitation assay [RIPA] buffer) and urea-soluble fractions from cBOs 7 days after blast exposure. Student’s t test, n = 4 in (B) and n = 5 in (C). Ponceau staining was used to normalize protein loading. (D–G) Representative WB and quantifications of STMN2 (D and E) and UNC13A (F and G) levels from total protein extracts of cBOs 7 days after blast exposure. GAPDH was used as loading control. Student’s t test, n = 8 in (E), n = 6 in (G). (H and I) Representative immunofluorescence images of TBI or sham cBOs 7 days after injury stained for UNC13A (green), TDP-43 (gray), and MAP2 (red). A representative MAP2-positive cell is shown in the inset, highlighting a shift in TDP-43 distribution from strictly nuclear in the sham control to cytoplasmic and aggregating after TBI. Magnification: 63x; scale bars: 15 μm in the main panels and 5 μm in zoomed in images. (J and K) Quantification of TDP-43 mean fluorescence intensity (MFI) in the nucleus ( n = 25) (J) and the correlation between normalized nuclear TDP-43 levels and normalized UNC13A levels (K). (L and O) Representative WB ( L ) and quantification of cryptic exon (CE)-containing HDGFL2 isoform ( n = 4) (M), HDGFL2 levels ( n = 6 for sham and n = 4 for TBI) (N), and their ratio ( n = 4) (O) in cBO 7 days after blast injury. GAPDH was used as loading control. For all, bars represent mean ± standard deviation, while circles and triangles indicate individual experiments using the two independent iPSC lines; ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001.

Journal: Cell Reports Methods

Article Title: A tabletop blast device for the study of the long-term consequences of traumatic brain injury on brain organoids

doi: 10.1016/j.crmeth.2025.101213

Figure Lengend Snippet: TBI-related neurodegenerative phenotypes emerge 7 days after injury of cBOs (A–C) Representative WB (A) and quantification of TDP-43 (B) and pTau (AT8, C) in detergent-soluble (i.e. radio-immunoprecipitation assay [RIPA] buffer) and urea-soluble fractions from cBOs 7 days after blast exposure. Student’s t test, n = 4 in (B) and n = 5 in (C). Ponceau staining was used to normalize protein loading. (D–G) Representative WB and quantifications of STMN2 (D and E) and UNC13A (F and G) levels from total protein extracts of cBOs 7 days after blast exposure. GAPDH was used as loading control. Student’s t test, n = 8 in (E), n = 6 in (G). (H and I) Representative immunofluorescence images of TBI or sham cBOs 7 days after injury stained for UNC13A (green), TDP-43 (gray), and MAP2 (red). A representative MAP2-positive cell is shown in the inset, highlighting a shift in TDP-43 distribution from strictly nuclear in the sham control to cytoplasmic and aggregating after TBI. Magnification: 63x; scale bars: 15 μm in the main panels and 5 μm in zoomed in images. (J and K) Quantification of TDP-43 mean fluorescence intensity (MFI) in the nucleus ( n = 25) (J) and the correlation between normalized nuclear TDP-43 levels and normalized UNC13A levels (K). (L and O) Representative WB ( L ) and quantification of cryptic exon (CE)-containing HDGFL2 isoform ( n = 4) (M), HDGFL2 levels ( n = 6 for sham and n = 4 for TBI) (N), and their ratio ( n = 4) (O) in cBO 7 days after blast injury. GAPDH was used as loading control. For all, bars represent mean ± standard deviation, while circles and triangles indicate individual experiments using the two independent iPSC lines; ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001.

Article Snippet: UNC13A, rabbit , Proteintech Group , 68483-1-Ig; RRID:AB_3085194.

Techniques: Radio Immunoprecipitation, Staining, Control, Immunofluorescence, Fluorescence, Standard Deviation